“Cracking” Cellular Metabolism: from Novel Sample Prep to Powerful Insights with Discovery-based LC/MS Workflows

Presenters:

Genevieve Van de Bittner, PhD
Scientist, Agilent Research Laboratories,Agilent Technologies, Inc.

Mark Sartain, PhD
LC/MS Applications Scientist, Agilent Technologies, Inc. 

Abstract

In this Webinar, we will demonstrate comprehensive untargeted metabolomics and lipidomics workflows from sample prep to data analysis within the context of cancer cell biology. We describe an innovative cellular extraction method that lyses cells and quenches metabolism under room temperature conditions. Additionally, we explore extending and automating this method with the Agilent Bravo Liquid Handling Platform to sequentially extract both polar metabolites and lipids from a single sample of harvested cells. Extracts are analyzed with an Agilent1260 Infinity II Prime LC and 6546 LC/Q-TOF system that offers simultaneous high mass resolution (>30k for 118 m/z) and wide linear dynamic range (~ 4 orders). Finally, we use a full suite of recent software introductions (Lipid Annotator) and enhancements (Profinder, MPP, Quant) to elucidate the molecular response to perturbations in a cancer cell model.

"Leveraging Global Metabolomics at the Intersection of Physiology and Behavior"

Moderator:

Samuel Bohney, Ph.D.
Business Development Executive, Midwest

Presenters:

Nicole Vike, Ph.D.
Post-Doctoral Fellow, Northwestern University - Feinberg School of Medicine, Department of Psychiatry and Behavioral Sciences

Sumra Bari, Ph D.
Post-Doctoral Fellow, Northwestern University-Feinberg School of Medicine, Department of Psychiatry & Behavioral Sciences

Abstract

Metabolomics offers researchers the ability to get closer to the phenotype by identifying small molecules and combining these results with bioinformatic and biochemical expertise, producing actionable insights. Metabolon’s Precision Metabolomics™ LC-MS global metabolomics platform provides a high-fidelity, reproducible analysis of the current-state of a biological system to reveal changes in key biological pathways.

As the interest in and understanding of sports-related head injuries continues to grow, Nicole Vike and Sumra Bari will share small molecule insights to better characterize these injuries. Drs. Vike and Bari have integrated metabolomic data with other technologies such as resting state fMRI, transcriptomics and computational behavior using a virtual reality task, to assess effects of exposure to head acceleration events (HAEs). These findings not only provide exciting insights into the mechanism and monitoring of HAEs, but also provide examples of integration of metabolomics with other data streams to fuel physiological discoveries.

Webinar attendees will have a chance to win a $100 Amazon Gift Card (three card giveaway)

"Measuring the metabolome: Using Cyclic IMS, Targeted pathway analysis, and novel chemistries to increase coverage, precision, and accuracy"

Presenters:

David Heywood
Senior Manager, Discovery Omics

Andrew Peck
Senior Manager, Targeted Omics

Abstract

Metabolomics and lipidomics continue to gain interest as differentiated methodologies in revealing the complexities of normal and pathological biology. The broad number and diversity of metabolites, and the interactions amongst them, demands the highest fidelity, accuracy, and precision in analytical measurements. Whether you are analyzing the global metabolome or specific classes or pathways, Waters has tools to ensure rigorous and reproducible quantitation. In this workshop, we will present on the game-changing SELECT Series Cyclic IMS mass spectrometer and the use of variable ion mobility resolution for metabolomics research, a suite of plug-and-play tools for targeted workflows, and new column chemistries developed for difficult to chromatograph metabolites and lipids.

“The TruQuant Workflow combined with Authentic compound libraries… unparalleled ID, QUANT and QC”

Abstract

What do we look for in a metabolomics Workflow? One of the biggest concerns we continuously battle is reproducibility. A typical experiment produces thousands of metabolic features. The Workflow should provide a means to differentiate these metabolic features (fragments, adducts, noise), provide data handing and analysis solutions including Q/C, identification, and removal of variances so that quantitation is not only accurate but reproducible.

"Achieving High Quality Data for High Quality Results in Metabolomics"

Host:

Amanda Souza
Metabolomics Program Manager, Thermo Fisher Scientific

Special Guests:

Dr. Kim Ekroos
Founder & CEO of Lipidomics Consulting Ltd.

Dr. Warwick Dunn
Professor of Analytical and Clinical Metabolomics, School of Biosciences, University of Birmingham, UK

Abstract

For metabolomics to provide valuable biological insights, robust reproducible measurements and confident metabolite identifications are required. During this interactive workshop we will explore critical steps before, during, and after LC/MS acquisition to ensure high quality metabolomics data. Please join us for a lively discussion with leaders in the field to learn how to avoid common pitfalls and optimize experimental design.

"Improving your confidence; how highly reproducible NMR & highly confident 4D-MS annotations can advance your metabolomics research Applications"

Host:

Lucy Woods, PhD
Business Unit Manager Phenomics and Metabolomics, Bruker Daltonics

Presenters:

Amy Freund, Ph.D.
Senior Applications Scientist II / Applications Development Product Manager Bruker BioSpin

Sven Meyer, Ph.D.
Senior Scientist · Solutions Development OMICs
Bruker Daltonik GmbH

Eduardo Nascimento, Ph.D.
Field Application Scientist – AIC (Applied, Industrial & Clinical MR Market Division) Bruker BioSpin Corp.

Abstract

NMR is an intrinsically quantitative analytical tool highly suited to metabolomic analysis. A single NMR spectrum can describe the physiological state of the individual; from small metabolites to larger lipo-proteins, and many, many compounds in between. That same NMR spectrum can deliver quantitative information of both known and unknown metabolites. With careful attention to SOP’s, high equality spectral data can be acquired, and compared with other spectra from labs around the globe. With access to spectra and metadata from many metabolomics projects, we can build models and develop comprehensive databases.

Additionally, the annotation of lipids can be very demanding due to the high number of structural variations. Mass spectrometry-based identification typically relies on characteristic fragments from lipid headgroups and side chains obtained from MS/MS experiments. Depending on the quality of the MS/MS spectra , the depth of the structure elucidation can cover different levels like molecular formula level, chain composition level, etc. While the matching of MS/MS spectral libraries such gives a broad and quick overview on the lipid content, the detail of the annotation level can be too high.

This workshop will focus on demonstrating the reproducibility of NMR and the versatility of the 1D proton spectrum for both targeted and non-targeted analysis. The complete suite of tools Bruker brings to your metabolomic project including hardware, software, and standardized solutions will be outlined. Furthermore, novel tools embedded within the MetaboScape MS software will be presented. By making use of selected fragmentation rules and by enabling visual investigation of retention time and Collisional Cross Section consistency within lipid groups, automatic identification can be used to simplify lipid annotation workflows and provide confident annotation within Bruker’s MetaboScape software package.

"Advances in Targeted Metabolomics: Applications in Parkinson’s Disease."

Presenter:

Katherine Black, Ph.D

Abstract

Tryptophan catabolism has an important role in orchestrating metabolic crosstalk between various tissues within the host, as well as between the host and its microbial inhabitants. Moreover, tryptophan and its downstream metabolites have been implicated as robust biomarkers for several clinical indications, and thus it is crucial to monitor the activity of this pathway when developing various therapeutic strategies. In this webinar event, we will highlight the power of biocrates’ standardized targeted metabolomics technology, with an emphasis on the newly launched Tryptophan metabolism assay and its potential to aid us in the fight against neurodegenerative disease.

During this event, you will learn:

• How targeted metabolomics enables absolute quantitation of biological metabolites with superior reproducibility, and thereby empowers the rapid discovery of deeper pathophysiological insights with direct clinical relevance.

• The advantages associated with a highly reproducible and reliable approach that offers exceptional comparability of analyte concentrations across unique metabolomic assays.

• The pivotal physiological function of the tryptophan metabolism pathway in health and disease, with a special focus on its role in monitoring treatment response of neurodegenerative disorders.